Research

The Qiu lab studies the evolution, mechanism and regulation of kinesin-14s using an interdisciplinary approach that integrates molecular biology, protein biochemistry, cell biology, structural biology, physics-based theoretical modeling, and single-molecule light microscopy. Notably, research from the Qiu lab has led to several important findings:

(1) We discovered the first context-dependent bidirectional kinesin-14 motor;

  • ❖Popchock, A.R.*, Tseng, K.-F.*, Wang, P., Karplus, P.A., Xiang, X., and Qiu, W.H.# (2017) The mitotic kinesin-14 KlpA contains a context-dependent directionality switch. Nat. Commun.,  8, 13999.

    (2) We, in collaboration with Prof. Bo Liu at UC Davis, discovered that OsKCH2 from the rice plant is the first kinesin-14 that autonomously exhibits processive minus-end-directed motility as a homodimer;

  • ❖Tseng, K.-F.*, Wang, P.*, Lee, Y.-R.*, Bowen, J., Gicking, A.M., Guo, L., Liu, B.#, and Qiu, W.H.#. (2018) The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor. Nat. Commun., 9:1067.


    (3) We discovered that in addition to the canonical role of enabling dimerization, the central stalk is also motility determinant of mitotic kinesin-14 motors;

  • ❖Wang, P.*, Tseng, K.-F.*, Gao, Y., Cianfrocco, M., Guo, L.J., and Qiu, W.H.# (2018) The central stalk determines the motility of mitotic kinesin-14 homodimers. Cur. Biol. In Press.